嘎丁沏崩竟椭赴辱惫唾炬炭伸
第一章 单元测试
1、
At the isoelectric point, the net charge of the protein molecule is 0.
A:对
B:错
答案: 对
2、
All the proteins have quaternary structure.
A:对
B:错
答案: 错
3、
The principle of salt precipitation, precipitation of proteins is ( )
A:Binding of proteins to form insoluble protein salt
B:To neutralize the charge and destroy the hydration shell
C:Lowering the dielectric constant of the protein solution
D:Adjust the isoelectric point of the protein solution
E:Denature proteins
答案: To neutralize the charge and destroy the hydration shell
4、
The main chemical bond that maintains the alpha-helical structure of the protein is( )
A:peptide bond
B:Hydrogen bonding between peptide bond atoms
C:Hydrogen bonding between side chains
D:Salt key
E:Disulfide bond
答案: Hydrogen bonding between peptide bond atoms
5、
The advanced structure of the protein depends on ( )
A:Hydrogen bonds in protein peptide chains
B:Peptide bonds in protein peptide chains
C:Amino acid composition and sequence in protein peptide chains
D:Peptide bond plane in protein peptide chain
E:Peptide units in protein peptide chains
答案: Amino acid composition and sequence in protein peptide chains
6、
The physical and chemical properties shared by proteins and amino acids are: ( )
A:Polymer properties
B:Colloidal properties
C:Amphoteric Dissociation
D:Precipitation properties
E:Denatured properties
答案: Amphoteric Dissociation
7、
The properties of denatured proteins are ( )
A:Solubility drops significantly
B:Loss of biological activity
C:Easy to be hydrolyzed by protease
D:Solidification
E:precipitation
答案: Solubility drops significantly;Loss of biological activity;Easy to be hydrolyzed by protease
8、
Protein denaturation is because ( )
A:Hydrogen bond is destroyed
B:Peptide bond cleavage
C:Subunit depolymerization
D:The hydration membrane is destroyed and the charge is neutralized
E:Disulfide bond cleavage
答案: Hydrogen bond is destroyed;Subunit depolymerization;Disulfide bond cleavage
9、
Primary structure of protein ( )
A:Is the basis of spatial structure
B:refers to amino acid sequence
C:It does not include disulfide bond
D:It mainly depends on peptide bond to maintain stability
E:α-helix and β-sheet are the major conformations of it
答案: Is the basis of spatial structure;refers to amino acid sequence;It mainly depends on peptide bond to maintain stability
10、
Which of the following are acid amino acids? ( )
A:Glutamate
B:Lysine
C:Arginine
D:Aspartate
E:Alanine
答案: Glutamate;Aspartate
第二章 单元测试
1、
Tm is directly related to the GC content of DNA and ionic strength.
A:对
B:错
答案: 对
2、
rRNA is the site for protein biosynthesis.
A:对
B:错
答案: 错
3、
In a DNA molecule, if the molar ratio of A is 17.2%, the molar ratio of C should be ( )
A:67.2％
B:32.8％
C:65.6％
D:17.2％
E:50.3%
答案: 32.8％
4、
The key part of DNA molecule to store and transmit genetic information is ( )
A:Pentose conformation
B:base rotation angle
C:base sequence
D:pentose phosphate skeleton
E:phosphate diester bond
答案: base sequence
5、
The force to maintain the lateral stability of DNA double helix is ( )
A:Base packing force
B:hydrogen bond between base pairs
C:Internal screw drainage
D:disulfide bond
E:phosphodiester bond
答案: hydrogen bond between base pairs
6、
What’s wrong with nucleic acid hybridization ( )
A:Two single stranded DNA from different sources can be hybridized as long as some bases are complementary
B:DNA single strand can hybridize with RNA strand with almost the same complementary base
C:RNA DNA hybrid chain can be synthesized by reverse transcription using mRNA as template
D:mRNA can be hybridized with the encoded polypeptide chain
E:Using molecular hybridization, the target gene can be fished from gene library
答案: mRNA can be hybridized with the encoded polypeptide chain
7、
The changes in DNA denaturation are: ( )
A:Hydrogen chain breaking and double helix structure breaking between chains
B:hyperchromic effect
C:Viscosity increase
D:covalent bond fracture
答案: Hydrogen chain breaking and double helix structure breaking between chains;hyperchromic effect
8、
The histones that make up the core particle of nucleosome are ( )
A:H1
B:H2A
C:H2B
D:H3
E:H4
答案: H2A;H2B;H3;H4
9、
As for the description of rRNA, it is correct to ( )
A:Large and small subunits that can combine with proteins to form ribosomes
B:It’s the most abundant RNA in the cell
C:Containing more rare bases
D:No secondary structure
E:Shortest half-life
答案: Large and small subunits that can combine with proteins to form ribosomes;It’s the most abundant RNA in the cell
10、
The biological functions of nucleotides in vivo include ( )
A:As cytoskeleton
B:Raw materials for nucleic acid synthesis in vivo
C:Some nucleotides can be used as second messengers of hormones
D:Some nucleotides are allosteric effectors of allosteric enzymes
E:Some nucleotides are the direct energy suppliers in the organism
答案: Raw materials for nucleic acid synthesis in vivo;Some nucleotides can be used as second messengers of hormones;Some nucleotides are allosteric effectors of allosteric enzymes;Some nucleotides are the direct energy suppliers in the organism
第三章 单元测试
1、
Vitamins are a class of high molecular organic compounds
A:对
B:错
答案: 错
2、
Too much or too little fat-soluble vitamin can cause disease
A:对
B:错
答案: 对
3、
Vitamin A deficiency can cause ( )
A:Night blindness
B:color blindness
C:cataract
D:chondropathy
E:albinism
答案: Night blindness
4、
The vitamins containing metal elements are ( )
A:Vitamin B1
B:vitamin B2
C:vitamin B6
D:vitamin C
E:vitamin B12
答案: vitamin B12
5、
The coenzymes of α – keto acids decarboxylase come from ( )
A:Vitamin B1
B:vitamin B2
C:vitamin B6
D:vitamin B12
E:vitamin C
答案: Vitamin B1
6、
As for the corresponding relationship between vitamins and their active forms, the wrong one is ( )
A:Vitamin B1-TPP
B:vitamin PP-CoA
C:vitamin B2-FAD
D:Folic acid-FH4
E:nicotinamide-NAD+
答案: vitamin PP-CoA
7、
The fat soluble vitamins are ( )
A:Vitamin A
B:vitamin B
C:vitamin C
D:Vitamin D
E:vitamin E
答案: Vitamin A;Vitamin D;vitamin E
8、
Vitamin D deficiency can cause ( )
A:cretinism
B:osteochondrosis
C:kidney stone
D:night blindness
E:rickets
答案: osteochondrosis;rickets
9、
Which of the following are the active forms of pantothenic acid ( )
A:FAD
B:NAD+
C:CoA
D:FH4
E:ACP
答案: CoA;ACP
10、
Which of the following statements about vitamins is wrong ( )
A:It is a kind of low molecular weight organic compounds
B:The human body cannot synthesize itself
C:Tissue components that do not form the body
D:Oxidizable energy supply
E:It can be used as coenzyme to participate in metabolism
答案: The human body cannot synthesize itself;Oxidizable energy supply
第四章 单元测试
1、
The higher the Km value of the enzyme, the lower the affinity to substrate.
A:对
B:错
答案: 对
2、
All the enzymes have coenzymes or prosthetic groups.
A:对
B:错
答案: 错
3、
The difference between coenzyme and prosthetic group is ( )
A:Different chemical essence
B:different physical and chemical properties
C:Different binding degree with enzyme protein
D:Containing different metal ions
E:different biological properties
答案: Different binding degree with enzyme protein
4、
What determines the specificity of enzymatic reactions is ( )
A:coenzyme
B:prosthetic group
C:enzyme protein
D:Whole enzyme
E:cofactor
答案: enzyme protein
5、
The mechanism of enzymatic reaction is ( )
A:reduce the activation energy of the reaction
B:reduce the free energy of the reaction
C:increase the free energy of the reaction
D:Increase the heat energy of the substrate
E:decrease the heat energy of the product
答案: reduce the activation energy of the reaction
6、
The inhibition of succinate dehydrogenase by malonic acid belongs to ( )
A:Irreversible inhibition
B:competitive inhibition
C:non competitive inhibition
D:Anticompetitive inhibition
E:feedback inhibition
答案: competitive inhibition
7、
Which of the following is true about Isoenzymes ( )
A:It can catalyze the same chemical reaction
B:generally, it is an oligoenzyme
C:It can exist in different tissues of the same individual
D:It has different physical and chemical properties
答案: It can catalyze the same chemical reaction;generally, it is an oligoenzyme;It can exist in different tissues of the same individual;It has different physical and chemical properties
8、
The function of the essential group in the enzyme molecule is ( )
A:Binding with substrate
B:maintaining the spatial conformation of enzyme
C:To change the substrate chemically
D:to accept protons or groups in a reaction
答案: Binding with substrate;maintaining the spatial conformation of enzyme;To change the substrate chemically;to accept protons or groups in a reaction
9、
Covalent modifications of enzymes include ( )
A:Phosphorylation and dephosphorylation
B:acetylation and deacetylation
C:Methylation and demethylation
D:adenosylation and deadenylation
答案: Phosphorylation and dephosphorylation;acetylation and deacetylation;Methylation and demethylation;adenosylation and deadenylation
10、
The characteristics of noncompetitive inhibition are ( )
A:The structure of inhibitor and substrate is not similar
B:the degree of inhibition depends on the concentration of inhibitor
C:The binding of the inhibitor to the active center of the enzyme
D:the binding of the enzyme to the inhibitor does not affect its binding to the substrate
答案: The structure of inhibitor and substrate is not similar;the degree of inhibition depends on the concentration of inhibitor;the binding of the enzyme to the inhibitor does not affect its binding to the substrate
第五章 单元测试
1、
Ubiquinone is not contained in any respiratory chain complex.
A:对
B:错
答案: 对
2、
Respiratory chain complex II is the coupling site of oxidative phosphorylation.
A:对
B:错
答案: 错
3、
The reduced form of flavin adenine dinucleotide is ( )
A:FADH
B:FAD
C:FADH++
D:FADH2
答案: FADH++
4、
What type of gradient is critical to ATP formation by oxidative phosphorylation?
A:sodium ion
B:potassium ion
C:chloride ion
D:proton
答案: proton
5、
Which of the following does not participate in, nor is a component of , the electron-transport chain?
A:coenzyme A
B:non-heme, iron-sulfur proteins
C:NADH
D:coenzyme Q
E:cytochrome c1
答案: coenzyme A
6、
In the malate-aspartate shuttle, electrons from NADH are transferred to , forming malate.
A:oxaloacelate
B:glutamate
C:acetate
D:aspartate
答案: oxaloacelate
7、
Choose the correct path taken by a pair of electrons as it travels down the electron-transport chain.
A:NADH→complex I→CoQ→complex III→ Cyt c→complex IV→O2
B:FADH2→complex I→CoQ→complex III→ Cyt c→complex IV→O2
C:NADH→complex I→complex II→complex III→ Cyt c→complex IV→O2
D:FADH2→complex II→CoQ→complex III→ Cyt c→complex IV→O2
答案: NADH→complex I→CoQ→complex III→ Cyt c→complex IV→O2;FADH2→complex II→CoQ→complex III→ Cyt c→complex IV→O2
8、
Factors affecting oxidative phosphorylation include: ( )
A:ADP / ATP
B:thyroxine
C:amiodarone
D:uncoupling agent
答案: ADP / ATP;thyroxine ;amiodarone;uncoupling agent
9、
ATP synthase: ( )
A:It is composed of two parts, F1 and F0
B:F0 is the proton channel of mitochondrial inner membrane
C:F1 catalyzes the production and release of ATP
D:F1 is the hydrophobic part of the enzyme
答案: It is composed of two parts, F1 and F0;F0 is the proton channel of mitochondrial inner membrane;F1 catalyzes the production and release of ATP
10、
The increase of basal metabolic rate in patients with hyperthyroidism is mainly due to: ( )
A:Increase of ADP decomposition
B:increase of ADP generation
C:Increased ATP decomposition
D:increased ATP production
答案: Increased ATP decomposition;increased ATP production
第六章 单元测试
1、
Aerobic oxidation is the major process to provide energy for most tissues.
A:对
B:错
答案: 对
2、
Anaerobic oxidation cannot be carried out under aerobic conditions.
A:对
B:错
答案: 错
3、
Which of the following is an energy-requiring step of glycolysis?
A:Lactate dehydrogenase
B:Phosphoglycerate kinase
C:Glucokinase
D:Phosphohexose isomerase
E:Pyruvate kinase
答案: Glucokinase
4、
Which of the following are primary substrates for gluconeogenesis?
A:Galactose and fructose
B:Glycerol and alanine
C:Acetyl-CoA and succinyl-CoA
D:Sucrose and lactose
E:GTP and biotin
答案: Glycerol and alanine
5、
When glycogen is synthesized, the direct donor of glucose group is ( )
A:CDPG
B:UDPG
C:glucose-1- phosphate
D:GDPG
E:glucose-6- phosphate
答案: UDPG
6、
How much ATP can be produced by 1-acetyl-coa through the TCA cycle?
A:10
B:12
C:14
D:16
E:18
答案: 10
7、
Which of the following pathway are only carried out in cytoplasm?
A:glycolysis
B:gluconeogenesis
C:pentose phosphate pathway
D:glycogen synthesis
答案: glycolysis;pentose phosphate pathway;glycogen synthesis
8、
The hormones that raise blood sugar are ( )
A:glucagon
B:adrenaline
C:glucocorticoid
D:parathyroid hormone
答案: glucagon;adrenaline ;glucocorticoid ;parathyroid hormone
9、
Approximately how many ATP can be produced during the completely oxidation of one glucose ( )
A:30
B:32
C:36
D:10
答案: 30;32
10、
The physiological functions of pentose phosphate pathway include ( )
A:supply pentose phosphate
B:supply 3C compounds
C:Provide 4C compounds
D:provide NADPH + H+
答案: supply pentose phosphate;supply 3C compounds;Provide 4C compounds;provide NADPH + H+
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